刘接卿
发表刊物：Phytotherapy Research
关键字：Physalis alkekengi L. var. franchetii; RNA-sequencing; antiinflammatory activity; withanolide.
摘要：In China, the fruits of Physalis alkekengi L. var. franchetii, which are conventionally utilized as edible berry, have attracted wide attention due to its significant biological activities. In the present study, phytochemical studies on the fruits of Physalis plants afforded six compounds, including two new withanolides (1-2) and four known agnologues (3-6). The inhibitory effects of these compounds on the formation of nitric oxide (NO) stimulated by lipopolysaccharide (LPS) in RAW264.7 macrophages were evaluated. Physapubescin M (1), with IC50 value of 1.58 μM, was selected for further study. The protein expression of COX-2 and iNOS, and LPS-induced production of cytokines (IL-6, IL-1β and TNF-α) were reduced by physapubescin M (1) in a dose-dependent way. In addition, transcriptomic analyses were conducted to profile gene expression alterations in LPS-induced RAW264.7 cells upon treatment of physapubescin M (1) and the potential antiinflammatory mechanism of withnolides was mentioned. These results provide broad view to the underlying antiinflammatory mechanism of withnolides, and give a theoretical basis for the utilization of the fruits of P. alkekengi L. var. franchetii.
论文类型：基础研究
是否译文：否
发表时间：2019-12-22
上一条：Wang, X.-H.#; Liu, J.-Q.#; Chen, S.Y.; Chen, S.; Yin, Y.F.; Liu, Y.; Zhang, C.H.*, Hydroxy-octadecenoic acids instead of phorbol esters are responsible for the Jatropha curcas kernel cake's toxicity. Commun Biol 2020, 3 (1), 228.
下一条：Xu, W.-J.; Xiao, Q.; Lian, C.-L.; Zhang, C.; Liu, J.-Q.*, The synthesis and cytotoxic activity of derivatives of 4β-hydroxywithanolide E. Steroids 2021, 166, 108776.
