所属单位：华中农业大学
发表刊物：MOLECULAR AND CELLULAR ENDOCRINOLOGY
刊物所在地：IRELAND
关键字：Chicken，Sex-biased miRNAs，miR-107，Gonadal development，NR5a1
摘要：Several studies indicate that sexual dimorphic microRNAs (miRNAs) in chicken gonads are likely to have important roles in sexual development, but a more global understanding of the roles of miRNAs in sexual differentiation is still needed. To this end, we performed miRNA expression profiling in chicken gonads at embryonic day 5.5 (E5.5). Among the sex-biased miRNAs validated by qRT-PCR, twelve male-biased and six female-biased miRNAs were consistent with the sequencing results. Bioinformatics analysis revealed that some sex-biased miRNAs were potentially involved in gonadal development. Further functional analysis found that over-expression of miR-107 directly inhibited nuclear receptor subfamily 5 group A member 1 (NR5a1), and its downstream cytochrome P450 family 19 subfamily A, polypeptide 1 (CYP19A1). However, anti-Mullerian hormone (AMH) was not directly or indirectly regulated by miR-107. Overall results indicate that miR-107 may specifically mediate avian ovary-development by post transcriptional regulation of NR5a1 and CYP19A1 in estrogen signaling pathways. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
论文类型：期刊论文
卷号：429
期号：C
页面范围：29-40
ISSN号：0303-7207
是否译文：否
发表时间：2016-03-17
收录刊物：SCI
DOI码：10.1016/j.mce.2016.03.033
影响因子：4.467
第一作者：苗楠
合写作者：侯岳,王馨
通讯作者：龚炎长,俸艳萍