所属单位：华中农业大学
发表刊物：Gene
刊物所在地：NETHERLANDS
关键字：Chicken，Gonadal development，miR-92，ATRX，DDX3X
摘要：MiR-17-92 cluster consists of multifunctional miRNAs related to gonadal development in mammals. Our preliminary data showed that gga-miR-92 was male-biased in chicken embryonic gonads at E5.5 and E6.5. MiR-92(a-2) and two putative targets (ATRX and DDX3X) were highly conserved and located on mammalian Chromosome X but on autosomes in chicken. Here, we studied the expression and interaction of miR-92 and the targets (ATRX and DDX3X) in chicken embryonic gonads. What's more, male-biased miR-92 shows an opposite expression tendency with ATRX and DDX3X in eight embryonic stages and different tissues at E10.5 by qRT-PCR. To verify the regulation relationship between miR-92 and two targets, we performed dual-luciferase reporter assay in DF1, overexpression and inhibition of miR-92 in chicken embryonic fibroblasts (CEFs). The results show that miR-92 directly targets ATRX and DDX3X by binding the 3' un-translated region (3'-UTR), and the over-expression and inhibition of miR-92 negatively regulates ATRX and DDX3X. After the identification of the expression of their downstream genes (AMH and WNT4) in mRNA level, we found that there is no regulatory relationship between ATRX and DDX3X. The overall results indicate that miR-92 may perform roles in early chicken gonadogenesis by regulating the expressions of ATRX and DDX3X, respectively.
论文类型：期刊论文
学科门类：理学
卷号：626
页面范围：326-336
ISSN号：0378-1119
是否译文：否
发表时间：2017-08-30
收录刊物：SCI
DOI码：10.1016/j.gene.2017.05.050
影响因子：3.913
第一作者：苗楠
合写作者：王馨
通讯作者：俸艳萍,龚炎长
发布期刊链接：https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(17)30406-7