Transcriptional responses of fluxapyroxad-induced dysfunctional heart in zebrafish (Danio rerio) embryos.
所属单位：华侨大学
发表刊物：Environmental Science and Pollution Research
刊物所在地：Germany
关键字：Fluxapyroxad；Zebrafish；Embryo；Heart malformation；Cardiotoxicity；Transcriptome
摘要：Fluxapyroxad (FLU) is a succinate dehydrogenase inhibitor (SDHI) fungicide used in controlling crop diseases. Potential toxicity to aquatic organisms is not known. We exposed zebrafish to 1, 2, and 4 μM FLU for 3 days. The embryonic zebrafish showed developmental cardiac defects, including heart malformation, pericardial edema, and heart rate reduction. Compared with the controls, cardiac-specific transcription factors (nkx2.5, myh7, myl7, and myh6) exhibited dysregulated expression patterns after FLU treatment. We next used transcriptome and qRT-PCR analyses to explore the molecular mechanism of FLU cardiotoxicity. The transcriptome analysis and interaction network showed that the downregulated genes were enriched in calcium signaling pathways, adrenergic signaling in cardiomyocytes, and cardiac muscle contraction. FLU exposure repressed the cardio-related calcium signaling pathway, associated with apoptosis in the heart and other manifestations of cardiotoxicity. Thus, the findings provide valuable evidence that FLU exposure causes disruption of cardiac development in zebrafish embryos. Our findings will help to promote a better understanding of the toxicity mechanisms of FLU and act as a reference to explore the rational use and safety of FLU in agriculture.
论文类型：基础研究
卷号：29
期号：60
页面范围：90034-90045
ISSN号：0944-1344
是否译文：否
收录刊物：SCI
DOI码：10.1007/s11356-022-21981-6
影响因子：5.19
第一作者：李文华
合写作者：郭珊珊
通讯作者：苗楠
发布期刊链接：https://link.springer.com/article/10.1007/s11356-022-21981-6