Associate professor
Supervisor of Master's Candidates
Gender:Female
Date of Birth:1988-11-07
Education Level:博士研究生
School/Department:华侨大学医学院
Business Address:华侨大学厦门校区泛华科技大楼B201
E-Mail:
Status:在岗
Other Post:药理学教研室主任
Discipline:Biomedical Engineering
Biomedical Engineering
Honors and Titles:
2024-07-25 全国大学生基础医学实验设计创新大赛(西部、中南部赛区) 三等奖 (指导老师)
2024-05-07 华侨大学第四届基础医学创新研究暨实验设计大赛 三等奖(指导老师)
2023-12-29 Brain Sciences 2023 Outstanding Reviewer Award
2024-04-14 医学院“精彩一堂课”三等奖
2023-06-30 2022-2023年 考核优秀
2019-06-30 2018-2019年 考核优秀
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Affiliation of Author(s):华中农业大学
Journal:Gene
Place of Publication:NETHERLANDS
Key Words:Chicken,Gonadal development,miR-92,ATRX,DDX3X
Abstract:MiR-17-92 cluster consists of multifunctional miRNAs related to gonadal development in mammals. Our preliminary data showed that gga-miR-92 was male-biased in chicken embryonic gonads at E5.5 and E6.5. MiR-92(a-2) and two putative targets (ATRX and DDX3X) were highly conserved and located on mammalian Chromosome X but on autosomes in chicken. Here, we studied the expression and interaction of miR-92 and the targets (ATRX and DDX3X) in chicken embryonic gonads. What's more, male-biased miR-92 shows an opposite expression tendency with ATRX and DDX3X in eight embryonic stages and different tissues at E10.5 by qRT-PCR. To verify the regulation relationship between miR-92 and two targets, we performed dual-luciferase reporter assay in DF1, overexpression and inhibition of miR-92 in chicken embryonic fibroblasts (CEFs). The results show that miR-92 directly targets ATRX and DDX3X by binding the 3' un-translated region (3'-UTR), and the over-expression and inhibition of miR-92 negatively regulates ATRX and DDX3X. After the identification of the expression of their downstream genes (AMH and WNT4) in mRNA level, we found that there is no regulatory relationship between ATRX and DDX3X. The overall results indicate that miR-92 may perform roles in early chicken gonadogenesis by regulating the expressions of ATRX and DDX3X, respectively.
Indexed by:Journal paper
Discipline:Natural Science
Volume:626
Page Number:326-336
ISSN No.:0378-1119
Translation or Not:no
Included Journals:SCI
DOI number:10.1016/j.gene.2017.05.050
Impact Factor:3.913
First Author:苗楠
Co-author:王馨
Correspondence Author:俸艳萍,龚炎长
Links to published journals:https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(17)30406-7
Pre One:Hippocampal MicroRNAs Respond to Administration of Antidepressant Fluoxetine in Adult Mice.
Next One:Identification of male-biased microRNA-107 as a direct regulator for nuclear receptor subfamily 5 group A member 1 based on sexually dimorphic microRNA expression profiling from chicken embryonic gonads